ABSTRACT

Background/Objectives:
Tea and coffee, two of the most widely consumed beverages worldwide, play important roles in supporting overall health. Changes in the urine proteome reflect the changes in the body influenced by beverage consumption, rather than beverage metabolites. In this study, the effects of teas with different fermentation levels and black coffee on the body were explored via urine proteomics analysis.

Methods:
Urine samples were collected from rats before and after seven consecutive days of consuming green tea, oolong tea, black tea, Pu-erh tea, or black coffee. Both before-and-after comparisons and between-group comparisons were performed, and the samples were analyzed using liquid chromatography coupled with tandem mass spectrometry.

Results:
The urine proteome reflected the changes in rats after consumption of teas or black coffee for one week. Biological processes and pathways enriched with differential proteins included fat cell differentiation, lipid metabolism, glucose metabolism, fatty acid transport, and immune response. The effects of teas with different fermentation levels and black coffee on the body exhibited a high degree of specificity. Additionally, several identified differential proteins have been reported as biomarkers for diseases such as cancer and cardiovascular diseases. This suggests that beverage consumption, including tea and black coffee, should be considered in urine biomarker research. And the use of biomarker panels may be necessary to improve accuracy.

Conclusions:
The urine proteome provides a comprehensive and systematic reflection of the effects of all components in teas and black coffee on the body and allows for the distinction of changes in the body after consumption of teas with different fermentation levels and black coffee.

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